Mixed / Miscellaneous Topics MCQs

Flow cytometry helps in subtyping hematopoietic cells. Blood cell counters give differential leukocyte count Coagulometers give coagulation profile Antibody quantification is usually done by ELISA Reference: Pathology Outlines - Flow cytometry

Flow cytometry is used routinely in the classification of leukemias and lymphomas. In this method, fluorescently labeled antibodies against cell surface molecules and differentiation antigens are used to obtain the phenotype of malignant cells. Flow cytometry is a technology that rapidly analyzes single cells or particles as they flow past single or multiple lasers while suspended in a buffered salt-based solution. Each particle is analyzed for visible light scatter and one or multiple fluorescence parameters. Reference: Robbins and Cotran Pathologic Basis of Diseases, 10 th ed, Pg: 238.

The most common method for large-scale analysis of RNA expression in use in research laboratories has been based on DNA microarrays. DNA microarray analysis helps as the bacterial genomic DNA often outlasts the viability of the bacteria and a diagnosis can be made using a small amount of DNA, as opposed to the large numbers of bacteria needed for culture. Reference: Robbins and Cotran Pathologic Basis of Diseases, 10 th ed, Pg: 240.

10% buffered neutral formalin is used in histopathology as fixative. Reference: Ackerman, 9th ed, Pg: 27